Inclusion Criteria:

• * Male or Female aged 18 or older
• * Second-degree burn wounds of less than 20% total body surface area (TBSA)
• * No active wound infection at screening based on clinical criteria
• * Able to provide written informed consent

Exclusion Criteria:

• * Wounds determined by study clinicians to be ineligible, including those that may inhibit access to the spleen for the ultrasound procedure
• * Participating in another research study that may affect the conduct of results of this study
• * BMI \> 30
• * Having or exhibiting any of the following:
• * Previous surgery of the spleen, esophagus, stomach, duodenum, or liver, including splenectomy
• * End stage renal disease and/or uremia
• * Active malignancy
• * Previous leukemia and/or lymphoma
• * Human immunodeficiency virus infection or AIDS
• * Rheumatoid arthritis or other immune-mediated diseases (e.g., inflammatory bowel disease
• * Arrythmias, including but not limited to, atrial fibrillation, atrial flutter, clinically significant bradycardia, ventricular arrhythmias, and A-V block
• * Implanted pacemaker or cardioverter/debribrillator
• * History of unstable angina, angioplasty or coronary arterial by-pass grafting surgery
• * History of stroke or TIA
• * Untreated thrombosis or bleeding disorders
• * Currently implanted vagus nerve stimulator
• * Currently implanted spinal cord stimulator or other chronically implanted electronic device
• * Receiving oral, topical, rectal, or parenteral corticosteroids in an unstable dose 4 weeks prior to baseline visit
• * Any immunosuppressive or cytotoxic medications
• * Clinically relevant history of alcohol or drug abuse as determined by the investigator including:
• * alcohol consumption within 4 days of the baseline visit
• * tobacco or nicotine product use within the past 1 month
• * recreational drug use within the past 1 month
• * Pregnant or breast feeding
• * Unable or unwilling to comply with study procedures
• * Have recently (within 3 months) been diagnosed with COVID, or have ever been diagnosed with long COVID
• * Have an autoimmune disorder, such as, rheumatoid arthritis or lupus