Inclusion Criteria:

• * Ages 20-80y
• * Lean and overweight (BMI 18.5-40 kg/m2)
• * No acute, chronic, or debilitating medical conditions
• * No prescription/non-prescription medications or drugs of abuse
• * Limited weight training or intense exercise (swimming, CrossFit)
• * Non-smoker
• * Average level of daily physical activity (8,000-12,499 steps/day)
• * Persons who fit all the above criteria and are suitable based on a medical history and health habits questionnaire, sleep profiling questionnaire, and electrocardiogram and clinical biochemical screening tests of blood and urine may be eligible to participate.

Exclusion Criteria:

• * Persons with any acute, chronic, or debilitating medical condition except pre-hypertension and/or mild to moderate sleep apnea will be excluded.
• * Persons with any symptoms of acute or active illness (e.g. fever, leukocytosis) will be excluded.
• * Persons with a history of severe psychiatric illness or psychiatric disorders will be excluded.
• * Persons with a history of regular night/or rotating shift work, or who have traveled more than three time zones during the one month prior to the study will be excluded.
• * Pregnant persons, decisionally impaired adults, and prisoners will be excluded.