Inclusion Criteria:

• 1. The subject is male or female, aged ≥21 and \<50.
• 2. The subject is in good general health with no evidence of any medical problems.
• 3. The subject is fluent in both written and spoken English.
• 4. The subject has provided informed consent and is willing to comply with the study procedures.

Exclusion Criteria:

• 1. The subject is obese (BMI\>30).
• 2. The subject has a known history of heart disease, lung disease, kidney or liver disease.
• 3. Diagnosis of asthma, sleep apnea, or use of CPAP.
• 4. Subject has diabetes.
• 5. Subject has a clotting disorder.
• 6. The subject a hemoglobinopathy or history of anemia, per subject report or the first blood sample, that in the opinion of the investigator, would make them unsuitable for study participation.
• 7. The subject has any other serious systemic illness.
• 8. The subject is a current smoker.
• 9. Any injury, deformity, or abnormality at the sensor sites that in the opinion of the investigators' would interfere with the sensors working correctly.
• 10. The subject has a history of fainting or vasovagal response.
• 11. The subject has a history of sensitivity to local anesthesia.
• 12. The subject has a diagnosis of Raynaud's disease.
• 13. The subject has unacceptable collateral circulation based on exam by the investigator (Allen's test).
• 14. The subject is pregnant, lactating or trying to get pregnant.
• 15. The subject is unable or unwilling to provide informed consent, or is unable or unwilling to comply with study procedures.
• 16. The subject has any other condition, which in the opinion of the investigators' would make them unsuitable for the study.