Inclusion Criteria:

• 1. Pediatric subjects (\<21 years of age)
• 2. \> 20 kg in weight;
• 3. Capable and willing to provide parental Informed Consent and patient Assent;
• 4. Acceptable candidate for laparoscopic surgery;

Exclusion Criteria:

• 1. Active cutaneous infection or inflammation;
• 2. Pre-existing immunodeficiency disorder and/or chronic use of systemic steroids;
• 3. Uncontrolled diabetes mellitus
• 4. Known, significant history of bleeding diathesis, coagulopathy, von Willebrand's disease or current platelet count \< 100,000 cells/mm3, baseline INR(international normalized ratio) ≥1.8, or fibrinogen level less than 150 mg/dl (if received a fibrinolytic agent within prior 24 hours);
• 5. Severe co-existing morbidities having a life expectancy of less than 30 days;
• 6. Currently involved in any other investigational clinical Studies;
• 7. Significant anemia with a hemoglobin level less than 10 g/dL or a hematocrit less than 30%;
• 8. Females who are pregnant, planning to become pregnant within 3 months of the procedure, or lactating;
• 9. Extreme morbid obesity (BMI greater than 45 kg/m2)
• 10. Patients presenting with Ascites